V5 his tag. Cloning into pcDNA™3.

V5 his tag His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) 1ml: V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) 200μl: 1168. Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. Lane 2: 293T cells transfected with a V5 tag-Chromobox protein homolog 1 expression vector containi a His-tag whole cell lysate at 10 µg Learn about epitope tags, their use in immunoassays, plus browse epitope tag products like antibodies to targets: C-Myc, HA tag, DYKDDDDK, and V5 tag. CT. The pIB/V5-His TOPO™ box contains 200 ng of linearized and topoisomerase I-activated pIB/V5-His-TOPO™ vector, dNTPs, 10X PCR buffer, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, salt solution, and pIB/V5-His/CAT. For other reading frames, use pMIB/V5-His B or pMIB/V5-His C. Plasmid pIZ-EGFP-MYO10-HMM from Dr. Store Box 1 at -20°C. The TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pEF6/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control templates and primers, primers for sequencing or PCR screening, and an expression control plasmid. Protein Tag: His Tag (6x), V5 Epitope Tag: Quantity: 1 Oct 5, 2011 · The His tag is commonly added at either the N- or C-terminus of a protein without interfering with its normal structure and function. p3xFLAG-CMV-8. Easy to clone into other vectors. Sources. 35 mg 293T cells transfected with a human CBX expression vector containing a myc-His-V5-tag, whole cell lysate with ab314555 at 1/30 dilution (2ug in 0. See pages 4–6 to develop a cloning strategy. Flow cytometry analysis of non-transfected CHO cells (a) and V5 fusion gene transfected CHO cells (b) using THETM V5 Antibody, mAb, Mouse (GenScript, A01724). Plasmid pcDNA3. Tag 1 6X His, V5 Bacterial Resistance Ampicillin Selectable Marker Zeocin Notes Vector for expressing C-terminally V5- and 6xHis-tagged proteins in mammalian cells. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a backbone of pYES2, offers an N-terminal tag. pTRE2pur. Restriction sites are labeled to indicate the cleavage site. 1/V5-His /LacZ 8433 bp 400 800 1200 1600 2000 2400 2800 3200 3600 4000 4400 4800 5200 5600 6000 6400 6800 7200 7600 8000 8400 Ac5 promoter lacZ V5 tag 6xHis SV40 poly(A) signal ori AmpR AmpR promoter The pcDNA3. The signal was developed with V5-mFABP4-his Transfection293 mFABP4-V5-his Transfection293 Beads SBIAnti-V5TagImmunomagneticBeads-30μL WBdetectionantibody Anti-V5TagAntibody,RabbitPAb(100378-T36)at1μg/mL Gel 13%SDS-PAGEreducinggel Secondaryantibody Dylight800-labeledantibodytorabbitIgG(H+L),at 1:10000dilution Observedbandsize 15kDa Type of tag Sequence/size Description; HA: Peptide tag: YPYDVPDYA: A highly immunoreactive epitope and can be used to purify tagged target proteins; can be used for co-IP studies and western blots. Using the V5-tag in combination with a His-tag is sometimes recommended. Product The PMT/BiP/V5-His A vector is a laboratory equipment product designed for protein expression and purification. V5 tag 1009 . pGene/V5-His offers several features that facilitate expression analysis and purification of recombinant proteins in mammalian cells: • C-terminal V5 epitope tag for detection with Anti-V5 Antibodies Choice of an epitope tag depends on many factors, but downstream application is the most important one. pHom-Mem1. 1/V5-His TOPO TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3. pCMV6-AN-FC. 1/V5-His C. 00元 The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. Select transformants on LB plates containing 50 to 100 μg/mL ampicillin (or 50 μg/mL blasticidin). 1/V5-His/lacZ vectors are qualified by restriction enzyme •C-terminal V5-6x His tag for easy detection and rapid purification •Cytomegalovirus (CMV) promoter for high-level expression •attR sites for Gateway™ cloning, enabling recombination with attL-flanked fragments •Neomycin resistance gene for stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. , 1988). pTracer-EF V5-His C. The multiple cloning site has been confirmed by gate and maintain the pMT/V5-His and pMT/V5-His/lacZ vectors, we recommend that you use the supplied 0. pTandem-1. Die TOPO™ TA-Box enthält alle für die Ligation erforderlichen Reagenzien, einschließlich 200 ng des Topoisomerase I-aktivierten pEF6/V5-His-TOPO™-Vektors, steriles Wasser, dNTPs, 10x PCR-Puffer, Salzlösung, Kontroll-Templates und -Primer, Primer für Sequenzierung oder PCR-Screening und ein Expressionskontrollplasmid. Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. D2. Explore the Scientific R&D Platform Scientific intelligence platform for AI-powered data management and workflow automation The InsectSelect pIB/V5-His Vector Kit includes 20 μg each of supercoiled lyophilized pIB/V5-His and pIB/V5-CAT vectors, and 2 μg lyophilized OplE2 Forward and Reverse sequencing primers. Eighteen bases coding six histidines are inserted right after the START codon or right before the STOP codon. Each antibody is validated for use in various applications. For other reading frames, use pMT/BiP/V5-His A or pMT/BiP/V5-His C. pEF6 V5-His A. It is also known as a hexa histidine-tag, 6xHis-tag, or His6 tag. As a result of its comparably great cost-benefit ratio, it is known as one of the most widely used protein affinity tags to date. May 28, 2024 · For example, the 14-amino-acid V5 tag, derived from simian virus 5 (SV5), provides a reliable way to detect target proteins. Topoisomerase activation of the pcDNA3. 4 (at 25°C) 1 mM EDTA 2 mM DTT 0. 1/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, GAL1 Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression pCMV-Tag 1. V5 tag 1013 . 4 ) in 1991 ( Lilius et al The pcDNA5/FRT/V5-His TOPO® TA Expression Kit is shipped on dry ice. Each kit contains a box with pSecTag/FRT/V5-His TOPO TA Cloning® reagents (Box 1) and a box with One Shot® TOP10 competent cells (Box 2). The pYES2/CT, pYES3/CT and pYES6/CT vectors (Figures 4 and 5) contain a C-terminal V5-His tag. 1/V5-His TOPO® TA Expression Kits is provided below. No single epitope tag is useful for every application. V5 tag 930 . {RE} Insect cell vector with a GFP-Zeocin™ resistance fusion gene, for constitutive expression of proteins with an optional C-terminal V5-6xHis tag. pMIB/V5-His is a 3. 6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five insect cells. It contains the Xpress™ epitope for simple detection with Invitrogen’s Anti-Xpress™ Antibody and a polyhistidine (6xHis) tag for purifi-cation using nickel resin. ALCAM. 克隆方法：多克隆位点，限制性内切酶. Skip to main content Support: 1-888-506-6887 The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. There are two sizes of the V5 tag ranging from 9–14 amino acids, although the longer tag is usually used. All lanes: Western blot - Anti-V5 tag antibody [EPR27187-61] (ab309485) at 1/1000 dilution. Select transformants on LB agar plates containing 50 to 100 μg/mL ampicillin. 0 20 µg of pAc5. V5 tag antibody in western blot. 1/V5-His vector also offers the following features: Small size (5. This is a Protein A gene fusion vector system based on two synthetic IgG-binding domains (ZZ) of Staphylococcus aureus Protein A, which has been used for extracellular expression of secretory proteins pAc5. Each kit contains a box with pcDNA5/FRT/V5-His TOPO TA Cloning® reagents (Box 1) and a box with One Shot® TOP10 competent cells (Box 2). pCMV-Tag 1. pcDNA6 V5-His B. Arguably the simplest affinity tag is the polyhistidine (His) tag. Pavel Krejčí's lab contains the insert human EGFR receptor tyrosine kinase, full length, wildtype and is published in Elife. Sequence Author : Thermo Fisher (Invitrogen) Jedes pEF6//V5™-His TOPO TA-Expressionskit enthält zwei Boxen. pFN22K HaloTag CMVd1. 1/V5-His A or pcDNA™3. 1 V5-His-TOPO (linearized) Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and expression of tagged proteins in yeast cells. Like other affinity tags, a His tag can be fused to either the N- or C-terminus of a protein. Be Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. E16-08-0583. Invitrogen. pAc5. Sf9 cells are supplied with GIBCO™ Grace's Insect Media, High Five™ Cells are supplied with non-transfected CHO cells (a) or His-tagged protein transfect-ed CHO cells (b) Using THETM His Tag Antibody [iFluor 488], mAb, Mouse (A01800-100, 4 µg/ml). pYES2. Used in different immunoassays as well as affinity purification Mainly used [16] TC tag, a tetracysteine tag that is recognized by FlAsH and ReAsH biarsenical compounds (CCPGCC) Ty tag (EVHTNQDPLD) V5 tag, a peptide recognized by an antibody The polyhistidine- or His6-tag is a protein tag originally developed for efficient protein purification in 1988 (Hochuli, Bannwarth & Döbeli et al. Die Topoisomerase-Aktivierung des pcDNA3. 1050 = 42 bp 14 amino acids = 1. coli strain like TOP10, DH5α™-T1R, JM109, or equivalent. Dec 25, 2023 · Horn V et al, for example, expressed RNF168-RING domain constructs in the petNKI-His-SUMO2-kan vector and purified using a His-SUMO tag and cleaved the tag using His-tagged SENP2 protease . pcDNA4 His B. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide. 1/V5-His A. Product Qualification The pAc5. Small and unlikely to affect function, His-tagged proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Cloning into pcDNA™3. Catalog Number V412020 Stable Unspecified Constitutive Inducible Viral/Non-Viral Nonviral A Milieu Molecule for TGF-beta Required for Microglia Function in the Nervous System. HIS-Tag本身的特性對目的蛋白沒有影響，不會形成二聚體；分子量較小，只有0. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a The InsectSelect™ Kit includes 20 μg each of supercoiled, lyophilized pIZ/V5-His and pIZ/V5-His/CAT, 2 μg of lyophilized OpIE2 Reverse Sequencing primer, 1 g Zeocin™, Cellfectin™ Reagent, and 1 L of medium for the insect cell line chosen. All reagents are guaranteed stable for 6 months when properly stored. 1/V5-His/lacZ, lyophilized in TE, pH 8. When choosing a tag, consider how the size and charge of the tag will ultimately affect the protein’s function, solubility and downstream analysis. 1/V5-His-TOPO™ vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. It contains a Protein A-Binding Immunoglobulin-Binding (PMT-Bip) tag and a V5 and 6xHis tag, which can be used for affinity purification and detection of the expressed protein. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a V5 tag was immunoprecipitated from 0. While the His-tag system is popular for its high yield and low costs, the time and effort it takes to establish successful His-tag purification is often neglected. 1/V5-His A Below is the multiple cloning site for pcDNA ™3. For other reading frames, use pcDNA™3. ickey TH et al expressed RIG-1 protein with a pET SUMO expression vector, with an additional serine residue between the SUMO tag and RIG-1 to improve tag A polyhistidine-tag, best known by the trademarked name His-tag, is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. C. For other reading frames, use pcDNA™4/myc-His B or pcDNA™4/myc-His C. 4 kDa. 1/V5-His TOPO™ TA Expressionskit bietet einstufiges Klonen von mit Taq amplifizierten PCR-Produkten in einen Vektor für hochstufige Expression. 质粒类型：哺乳动物表达载体. For other reading frames, use pcDNA™4/V5-His B or pcDNA™4/V5-His C. Product Qualification The pMT/BiP/V5-His A, B, and C and pMT/BiP/V5-His/GFP vectors are qualified by Aug 30, 2011 · Its C-terminal (6xHis) tag enables purificaton and detection. C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin. For other reading frames, use pCMV-Tag 5A or pCMV-Tag 5C. D5. 1/V5-His vectors also offer the following features: • Small size (5. 1/His B or pcDNA™3. This vector is intended for use in eukaryotic cell lines to facilitate the expression and secretion of recombinant proteins with a C-terminal V5-His tag. History. Note that the kit does not contain Taq polymerase. pHTC HaloTag CMV Tag 1 6X His, V5 Bacterial Resistance Ampicillin Notes Contains secretion tag Catalog Number K6025-01 Stable Stable Constitutive Constitutive Viral/Non-Viral Nonviral pEF6/V5-His TOPO TA Cloning® Reagents pEF6/V5-His TOPO TA Cloning® reagents (Box 1) are listed below. pcDNA™3. pii: mbc. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e. Note Taq polymerase. pCMV6-A-Hygro. 1 TOPO™ TA Expression Kit contains two boxes. Specifically, IPI researchers fuse the V5 tag to antibody sequences and transfect the constructs into yeast cells. The V5-tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and Nanobodies. 93 kDa). 0 to transform a recA, endA E. 载体抗性：Ampicillin (氨苄 Each pIB/V5-His TOPO™ TA Expression Kit contains two boxes. No blue/white clonal selection of transformants. 4 kb) to improve DNA yields and increase subcloning efficiency. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). TOPO TA Cloning® Reagents pcDNA5/FRT/V5-His TOPO TA Cloning® reagents (Box 1) are listed Compact insect cell vector with a Zeocin™ resistance marker, for constitutive expression of proteins with an optional C-terminal V5-6xHis tag. Slideshow 5533415 Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. 1/V5-His TOPO™ TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Explore the Scientific R&D Platform. pIB V5-His Insect cell vector for transient or stable constitutive expression of C-terminally V5-6xHis-tagged proteins. sALCAM. 1/V5-His B. Qin Y, Garrison BS, Ma W, Wang R, Jiang A, Li J, Mistry M, Bronson RT, Santoro D, Franco C, Robinton DA, Stevens B, Rossi DJ, Lu C, Springer TA. Relative detection of V5 tag was observed across different proteins fused with V5 tag in V5-H3-His (Lane 3) and Myc-p65-V5 (Lane 4-7), using V5 Tag Monoclonal Antibody (Product # R960-25) in Western Blot. The vector is supplied in three reading frames to facilitate cloning. 35mg lysates). Store at -20°C. Nov 23, 2024 · The poly (His) tag is a widely-used protein tag; it binds to metal matrices. The tag used for this work contains 31 residues including a 14-residue epitope, readily detectable by a sensitive anti-V5 antibody, and a stretch of histidines to facilitate purification with Ni 2+-chelating resins (hexa moiety, M r = 0. Store at –20°C. The V5 tag is derived from the P and V proteins of the paramyxovirus simian virus 5. Can use pCoHygro or pCoBlast for selection. To express your gene as a recombinant fusion protein, you must clone your gene in frame with the C-terminal V5-His peptide tag. 1-EGFR-V5/HIS from Dr. Product: epitope tag from simian Mar 19, 2011 · His Tagging of Connexins. 目录号 IPD8011. This plasmid is available through Addgene. 1054 = 42 bp 14 amino acids = 1. The control plasmid, pEF1/V5-His/lacZ, is the pEF1/V5-His A vector with a The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid residues 95-108 of the simian virus 5 RNA polymerase alpha subunit. Nov 11, 2013 · The mouse AMCase was expressed as a fusion protein with Protein A, a V5 epitope and a (His) 6 tag (V5-His)(Figure 1) using the pEZZ18 vector . Secretion of proteins into serum-free medium simplifies purification, making it easier to har Drosophila vector for inducible high-level expression of secreted and tagged proteins. Das pcDNA™3. {RE} Each pEF6/V5-His TOPO™ TA Expression Kit contains two boxes. 21536. 75 = 42 bp 14 amino acids = 1. 5 μg/μl stock solution in TE, pH 8. pii: e21536. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a Relative detection of V5 tag was observed across different proteins fused with V5 tag in Myc-p65-V5 (Lane 3-5) and V5-H3-His (Lane 6-7), using V5 Recombinant Mouse Monoclonal Antibody (TCM5), HRP (Product # 740058MHRP) in Western Blot. The pcDNA™3. 载体大小：5463 bp. 1/V5-His TOPO® TA Cloning® reagents (Box 1) are listed below. 1/V5-His-TOPO vector allows PCR products For other reading frames, use pCMV-Tag 2A or pCMV-Tag 2C. The pYES2. I designed primers with V5 sequence and tried to amplify pEF6 V5-His-TOPO (linearized) Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and expression of tagged proteins in mammalian cells. If the desired downstream application is recombinant protein purification, epitope tags like 6X-His, GST (Glutathione-S-transferase), and MBP (Maltose Binding Protein) are widely used in affinity purification. D3. For other reading frames, use pAc5. In addition, the GeneSwitch™ protein upregulates its own expression, leading to amplified expression of the gene of interest from pGene/V5-His. C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody. Store all reagent at -20°C. R960-25) at a 1:1000 dilution. TM. 载体名称： pUB6/V5 His A. Polyhistidine was first used to purify recombinant galactose dehydrogenase by immobilized metal affinity chromatography (IMAC; UNIT 9. For other reading frames, use pMT/BiP/V5-His A or pMT/BiP/V5-His B. 0 20 µg of pMT/BiP/V5-His/GFP, lyophilized in TE Buffer, pH 8. 1/V5-His and pAc5. Product: epitope tag from simian Mammalian expression vector for tagging proteins with a C-terminal Myc epitope. 84KDa，對蛋白的下游應用不會產生影響；免疫原性低，可將純化的蛋白直接注射動物進行免疫並製備抗體；HIS-Tag與細菌的轉錄轉譯機制兼容，有利於蛋白表達；與其他標籤構建雙標籤 Tag 1 V5 (Cterm), His (Cterm) Bacterial Resistance Ampicillin Notes Inducible insect cell expression. No. g. To date, most epitope tags such as myc, HA, V5 and FLAG tags are recognized by Contents 20 µg each of pAc5. pcDNA5 WB analysis of Recombinant protein using 14440-1-AP. The V5 epitope tag gives an additional means of detection by Anti-V5 Antibody, and the Enterokinase(EK) cleavage site enables removal of the N-terminal leader peptide. pcDNA6 V5-His A. It contains a CMV promoter, a BiP signal sequence, and a V5 epitope tag sequence. 5 hours. Western blot analysis of V5 tag was performed by loading 20 µg of whole cell extracts of untransfected HEK-293 and HEK-293 transiently overexpressing V5-His-LacZ. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a Insect cell vector for expression of secreted, C-terminally V5-6xHis-tagged proteins. The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. Catalog Number V86120 Stable Transient Constitutive Constitutive Viral/Non-Viral Nonviral The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. 0 Shipping/Storage Lyophilized vectors are shipped at room temperature and should be stored at -20°C. Consult the multiple cloning sites described on pages 3–5 to determine which vector (A, B, or C) should be used to clone your gene in-frame with the C-terminal V5 epitope and the polyhistidine tag. TOPO TA Cloning® Reagents pSecTag/FRT/V5-His TOPO TA Cloning® reagents (Box 1) are Mar 9, 2015 · pEF6/V5-His To propagate and maintain the pEF6/V5-His vectors, use a small amount of the supplied 0. Sequence Author : Thermo Fisher (Invitrogen) HA, His, DYKDDDDK, and V5 are commonly used epitope tags for affinity purification. 1/V5-His A, B, and C, lyophilized in TE, pH 8. pFC27A HaloTag CMV-neo. Drosophila vector for inducible high-level expression of secreted and tagged proteins. The multiple cloning site has been confirmed by Aug 31, 2020 · Epitope tagging is a versatile approach to study different proteins using a well-defined and established methodology. Be sure Contents 20 µg each of pMT/BiP/V5-His A, B, and C, lyophilized in TE Buffer, pH 8. Epitope tagging is widely used in proteomics and genetic studies. This product has been shown to detect V5 Tag at both N- and C- termini of a fusion protein. References T7-tag, an epitope tag derived from the T7 major capsid protein of the T7 gene (MASMTGGQQMG). The Bad. The kit is expensive. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a Mammalian vector for expressing proteins with a cleavable N-terminal 6xHis tag. Figure: Adding Polyhistidine Tags : This is an example of a primer designed to add a 6xHis-tag using PCR. The PMT-Bip-V5-His tag vector is a laboratory tool used for the expression and purification of recombinant proteins. Note that there is a stop codon between the BamH I site and the BstX I site. Lane 1: 293T cells transfected with an empty vector containi a His tag whole cell lysate at 20 µg. The pEF6/V5 To propagate and maintain the pMT/BiP/V5-His and pMT/BiP/V5-His/GFP vectors, we recommend using the supplied 0. 1/V5-His A or pAc5. Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:32000 incubated at room temperature for 1. pCMV6-AN-FC-S. 5 µg/µL stock solution in TE buffer, pH 8. His: Peptide tag: 6X-His HHHHHH tag: Widely used tag for purification of target proteins that can be used with almost all expression systems. Thomas Friedman's lab contains the insert MYO10 and is published in Mol Biol Cell. pcDNA3. B. Supplementary Figure 1. Store Box 2 at -80°C. 0 Shipping/Storage Lyophilized vectors are shipped at room temperature and should be stored at Œ20°C. pEF6/V5-His TOPO TA Cloning® Reagents pEF6/V5-His TOPO TA Cloning® reagents (Box 1) are listed below. 1/V5-His A Plasmid Type Mammalian Expression Promoter CMV Expression Level High Cloning Method Restriction Enzyme Size 5502 5' Sequencing 1 Primer CMV-F 3' Sequencing 1 Primer BGH-rev 5' Terminal C-Term 5' Terminal 2 C-Term Tag 1 V5 Tag 2 Myc Bacterial Resistance Ampicillin Selectable Marker Neomycin Notes Aug 27, 2021 · The pAc5. 1/V5-His TOPO® TA Expression 20 K4800-01 Kit 40 K4800-40 TOPO® TA Cloning® that the user must supply Reagents pcDNA™3. 1/V5-His-TOPO™ Vektors ermöglicht das Ligieren von PCR-Produkten in nur 5 Minuten auf Ihrer Arbeitsfläche und ergibt dabei 90 % Rekombinanten. 4 kb expression vector designed for use in Drosophila cells to • C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for Each pYES2. Product: epitope tag from simian The pSecTag/FRT/V5-His TOPO® TA Expression Kit is shipped on dry ice. All pcDNA vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification The pAc5. Sequence Author : Thermo Fisher (Invitrogen) V5 标签来源于副粘病毒猴病毒 5 的 P 和 V 蛋白。V5 标签的大小有两种，范围在 9-14 个氨基酸之间，但通常使用长一点的标签。有时建议将 V5 标签与 His 标签结合使用。注意事项: 交叉反应性：如果您正在使用哺乳动物表达系统，则有可能发生交叉反应。 pCMV-Tag 1. See all anti-V5 tag antibodies. . 1 TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pYES2. 1/V5-His A, pcDNA3. Product: FLAG epitope tag, followed by an enterokinase cleavage site: pTracer-EF V5-His B. The V5 tag is generally used with all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence. COS7). The His-tag (6xHis-tag, His6-tag or polyhistine-tag) is a popular affinity tag for protein purification since its small size ensures a low impact on protein structure. 1% Triton X-100 100 μg/mL BSA The InsectSelect pIB/V5-His Vector Kit includes 20 μg each of supercoiled lyophilized pIB/V5-His and pIB/V5-CAT vectors, and 2 μg lyophilized OplE2 Forward and Reverse sequencing primers. pTracer™-EF/V5-His A, B and C are C-terminal fusion vectors. Use the following outline to clone and express your gene of interest in pcDNATM4/V5-His. 75 = 42 bp Download pSecTag FRT V5-His-TOPO (linearized For other reading frames, use pcDNA™4/V5-His A or pcDNA™4/V5-His C. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a V5 tag 34 . 1/V5-His B or pcDNA™3. Our V5 tag antibodies are designed to reliably detect recombinant V5-tagged proteins. pEF5 FRT V5-DEST. 1/myc-His B or pcDNA™3. 971 = 42 bp 14 amino acids = 1. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a . 1/His C. 5' 测序引物及序列：T7 Fwd:5'd[TAATACGACTCACTATAGGG]3' 载体标签：His Tag (6x), V5 Epitope Tag. pcDNA 3. For other reading frames, use pcDNA™6/myc-His B or pcDNA™6/myc-His C. 2016 Dec 8. What to watch out for V5-tag is a short peptide tag for detection and purification of proteins. coli strain like TOP10, DH5α ™ -T1 R , or equivalent. 4 kb) to improve DNA yields and increase subcloning efficiency • C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin The polyhistidine affinity tag, also known as the His-tag or His 6, usually consists of six consecutive histidine residues, but can vary in length from two to ten histidine residues. D1. You can help EcoliWiki by writing about the history of this plasmid. Wild-type ALCAM. Meijers notes that IPI commonly uses the V5 tag in its own antibody discovery operation. pcDNA5 Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged proteins. pcDNA4 V5-His C. Kit Reactions Catalog no. 1/V5-His is a 5. pCMV6-AC-HA-His. 1 V5-His C Mammalian vector for expressing C-terminally V5- and 6xHis-tagged proteins. V5 and myc is a good choice, you also might want to consider making a construct that is N-terminally tagged and incorporating a protease site to give you a cleavable tag, where you can remove the For other reading frames, use pcDNA™6/V5-His B or pcDNA™6/V5-His C. The pYES2/CT vec- V5 tag 34 . doi: 10. Explore the Scientific R&D Platform Scientific intelligence platform for AI-powered data management and workflow automation Dec 3, 2024 · pUB6/V5 His A载体. 5 μg/μL stock solution in TE buffer to transform a recA, endA E. 4 kb expression vector designed for use in Drosophila cells to • C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for The pEF6/V5-His TOPO® TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO® Cloning. 1 Hygro(-) pCMV-Tag 2A. Reliably detect and purify V5 tagged target proteins without a protein-specific antibody or probe. The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). 1/myc-His C. Jan 8, 2007 · Quick one-step cloning; can be used for direct insertion of Taq polymerase-amplified PCR product with no need to ligate; product can be transformed into yeast; protein can be expressed with galactose promoter and purified via the V5/His Tag. V5-His-LacZ was detected at ~117 kDa using V5 Tag Monoclonal Antibody (Cat. 75 = 42 bp Download pSecTag FRT V5-His-TOPO (linearized The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. 1/V5-His A, B, and C, Continued Multiple Cloning Site of pcDNA™3. FLAG Oct 14, 2014 · A. 启动子：UbC. 2017 Feb 15;6. 1% Triton X-100 100 μg/mL BSA pAc5. D4. coli strain like TOP10F´, DH5 , JM109, or equivalent. V5-His tag. Types of Kits Ordering information for the pcDNA™3. For other reading frames, use pMT/V5-His B or pMT/V5-His C. Item Composition Amount pEF6/V5-His-TOPO® 10 ng/μL plasmid DNA in: 50% glycerol 50 mM Tris-HCl, pH 7. 7554/eLife. Product: epitope tag from simian virus 5: V5 tag 34 . izmrl fcnzv glxosdm ldxwzc qxzlt qjtjddij ezrlax kdwzo lsugu qauby llrs daiprn ybwzwc tqun rtv